That means the U.S. has now sent more than $1 billion in humanitarian aid since the two-year Syrian civil war started.
Obama is timing the new aid to coincide with Eid al-Fitr , a holiday marking the end of Ramadan, Islam’s holy month of fasting.
Obama says many Americans have celebrated Ramadan with Muslims. He says millions in Syria are displaced and that the U.S. wants to help.
The humanitarian aid follows recent approval of a U.S. aid package of weapons to Syrian rebels aimed at reversing recent battlefield gains by President Bashar Assad
The White House issued the statement Wednesday while Obama was traveling in California.